MYO5A Antibody

ProSci
Product Code: PSI-42-827
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-827-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-827 (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2 / 2

42-827 (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
MYO5A, myosin VA (heavy chain 12, myoxin), GS1, MYH12, MYO5, MYR12, dilute, myosin V, myosin VA, myosin, heavy polypeptide kinase, myoxin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 200kDa band observed in lysates of cell line Jurkat (calculated MW of 215kDa according to NP_000250.3). Recommended concentration: 1-3ug/ml.Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (R?der et al, Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2031-6; PMID: 20133847). Recommended concentration: 1-3ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: C-ETKQLELDLN
NCBI Gene ID #:
4644
NCBI Official Name:
myosin VA (heavy chain 12, myoxin)
NCBI Official Symbol:
MYO5A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000250.3, NP_001135967.1
Protein GI Number:
215982791
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1).
Swissprot #:
Q9Y4I1