Dynamin-1 Monoclonal Antibody

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ProSci
Product Code: PSI-32-146
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-146-0.1mL0.1mL£657.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 3G4B6
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Dynamin-1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using Dynamin1 monoclonal antibody against truncated Dynamin1 recombinant protein SKN - SH cell lysate and NIH / 3T3 cell lysate .
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Western blot analysis using Dynamin1 monoclonal antibody against truncated Dynamin1 recombinant protein SKN - SH cell lysate and NIH / 3T3 cell lysate .

Documents

Further Information

Additional Names:
DNMDNM
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000
Determining optimal working dilutions by titration test.
Background:
Dynamin-1 (Dyn1), with 864-amino acid protein (about 95 kDa), belongs to the dynamin family. Dynamin-1 (neuron-specific), dynamin-2 (ubiquitously expressed), and dynamin-3 (expressed only in the testis, brain, and lung), constitute the dynamin family. Members of the dynamin family are GPTase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin-1 is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C. Dynamin-1 is a large GTPase enzyme required in membrane constriction and fission during multiple forms of endocytosis. Dynamin-1 is also a key molecule required for the recycling of synaptic vesicles in neurons, and it has been known that dynamin-1 gene expression is induced during neuronal differentiation.
Background References:
  • Annie Quan and Phillip J. Robinson. Methods Enzymol. 2005; 404:556-69.
  • Jiyun Yoo, Moon-Jin Jeong, Byoung-Mog Kwon. J. Biol. Chem., Mar 2002; 277: 11904 ? 11909.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant Dynamin-1 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
1759
NCBI Official Name:
dynamin 1
NCBI Official Symbol:
DNM1
NCBI Organism:
Homo sapiens
Protein Accession #:
Q05193
Protein GI Number:
172046078
Research Area:
Neuroscience
Swissprot #:
Q05193
User NOte:
Optimal dilutions for each application to be determined by the researcher.