Lck Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-186-0.1mL | 0.1mL | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 8E5F9
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lck monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Leukocyte C-terminal Src kinase, LSK, YT16, p56lck, pp58lck
Application Note:
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Lck(lymphocyte-specific protein tyrosine kinase), with 509-amino acid protein (about 56 kDa), belongs to the Src non-receptor tyrosine kinases family. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Lck is expressed predominantly in T cells and is localized to the inner surface of the plasma membrane. Lck is activated after T cell stimulation and is required for T-cell proliferation and IL-2 production. Aberrant expression or activation of Lck kinase has been reported in both lymphoid and nonlymphoid malignancies. In addition, inhibition of Lck has been a target to prevent lymphocyte activation and acute rejection.
Background References:
- Mingjian Shi, John C. Cooper, Chao-Lan Yu. Mol. Cancer Res., Jan 2006; 4: 39?45
- Robert F. Stachlewitz, Michelle A. Hart, Brian Bettencourt. J. Pharmacol. Exp. Ther., Oct 2005; 315: 36?41.
- Ibrahim Y. Hawash, Kamala P. Kesavan, Anthony I. Magee. J. Biol. Chem., Feb 2002; 277: 5683?5691.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant Lck expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
3932
NCBI Official Name:
lymphocyte-specific protein tyrosine kinase
NCBI Official Symbol:
LCK
NCBI Organism:
Homo sapiens
Protein Accession #:
P06239
Protein GI Number:
125474
Research Area:
Signal Transduction ,Immunology
Swissprot #:
P06239
User NOte:
Optimal dilutions for each application to be determined by the researcher.