Vimentin Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-235-0.1mL | 0.1mL | £560.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 9E7E7
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Vimentin monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
HEL113, CTRCT30
Application Note:
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Determining optimal working dilutions by titration test.
Background:
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Background References:
- Seshadri, R., et al. Intl. J. Cancer 67: 353-356(1996).
- Essa, T.M., et al. J. Egyptian Soc. Parasitol. 26:433-442(1996).
- Chu, Y.W., et al. Amer.J. Pathol. 148: 63-69(1996).
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant Vimentin expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
7431
NCBI Official Name:
vimentin
NCBI Official Symbol:
VIM
NCBI Organism:
Homo sapiens
Protein Accession #:
P08670
Protein GI Number:
55977767
Research Area:
Neuroscience
Swissprot #:
P08670
User NOte:
Optimal dilutions for each application to be determined by the researcher.