PHR1 Antibody

ProSci
Product Code: PSI-46-974
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-974-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
46-974 (1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

46-974 (1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PLEKHB1, pleckstrin homology domain containing, family B (evectins) member 1, KPL1, PHR1, PHRET1, PH domain containing protein in retina 1, PH domain containing, retinal 1, evectin-1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 25kDa band observed in Rat Brain lysates (calculated MW of 25.2kDa according to Rat NP_742030.1 (Evectin-1) and of 27.2kDa according to Human NP_067023.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Andrews KL, Potdar PD, Nettesheim P, Ostrowski LE. KPL1, which encodes a novel PH domain-containing protein, is induced during ciliated cell differentiation of rat tracheal epithelial cells. Exp Lung Res. 2000 Jun;26(4):257-71.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Cow
Immunogen:
The immunogen for this antibody is: PPDSALESPFEE-C
NCBI Gene ID #:
58473
NCBI Official Name:
pleckstrin homology domain containing, family B (evectins) member 1
NCBI Official Symbol:
PLEKHB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_067023.1, NP_001123506.1, NP_001123505.1, NP_001123507.1, NP_001123508.1,
Protein GI Number:
10864013
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize reported isoforms a (NP_067023.1) and c (NP_001123505.1).
Swissprot #:
Q9UF11