PITX3 Antibody

ProSci
Product Code: PSI-46-197
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-197-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-197 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

46-197 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PITX3, paired-like homeodomain 3, MGC12766, PTX3, homeobox protein PITX3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 40kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 31.8kDa according to NP_005020.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Coulon et al, J Biol Chem. 2007 Nov 9;282(45):33192-200.; PMID: 17848564). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Kim J, Inoue K, Ishii J, Vanti WB, Voronov SV, Murchison E, Hannon G, Abeliovich A. A MicroRNA feedback circuit in midbrain dopamine neurons. Science. 2007 Aug 31;317(5842):1220-4.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: PEHGCKGQEHSD
NCBI Gene ID #:
5309
NCBI Official Name:
paired-like homeodomain 3
NCBI Official Symbol:
PITX3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005020.1
Protein GI Number:
4826912
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is not excpected to cross-react with PITX1 and PITX2.
Swissprot #:
O75364