PLRG1 Antibody

ProSci
Product Code: PSI-46-203
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-203-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-203 (0.1ug/ml) staining of HEK293 (A) and NIH3T3 (B) nuclear cell lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-203 (0.1ug/ml) staining of HEK293 (A) and NIH3T3 (B) nuclear cell lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
PLRG1, pleiotropic regulator 1 (PRL1 homolog, Arabidopsis), PRL1, MGC110980, pleiotropic regulator 1 (PRL1, Arabidopsis homolog), pleiotropic regulator 1 (PRL1homolog, Arabidopsis), pleiotropic regulator 1 (PRL1, Arabidopsis homolog)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in nuclear lysates of cell lines HEK293 and NIH3T3 (calculated MW of 57.2kDa according to Human NP_002660.1 and 56.9kDa according to Mouse NP_058064.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
Background References:
  • Ajuh P, Lamond AI Identification of peptide inhibitors of pre-mRNA splicing derived from the essential interaction domains of CDC5L and PLRG1 Nucleic Acids Res. 2003 Nov 1;31(21):6104-16
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-PVSWKPEIIKRKRF
NCBI Gene ID #:
5356
NCBI Official Name:
pleiotropic regulator 1
NCBI Official Symbol:
PLRG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002660.1, NP_001188493.1
Protein GI Number:
4505895
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_002660.1) and isoform 2 (NP_001188493.1).
Swissprot #:
O43660