POLQ Antibody
| Code | Size | Price |
|---|
| PSI-42-037-0.1mg | 0.1mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
POLQ , polymerase (DNA directed), theta , DKFZp781A0112, POLH, PRO0327, DNA polymerase eta, DNA polymerase theta
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments in Human Brain (Cerebellum lysates) and in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Background References:
- Kawamura K, Bahar R, Seimiya M, Chiyo M, Wada A, Okada S, Hatano M, Tokuhisa T, Kimura H, Watanabe S, Honda I, Sakiyama S, Tagawa M, O-Wang J. DNA polymerase theta is preferentially expressed in lymphoid tissues and upregulated in human cancers. Int J Cancer. 2004 Mar;109(1):9-16.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: DTHQQLTCSNDES
NCBI Gene ID #:
10721
NCBI Official Name:
polymerase (DNA directed), theta
NCBI Official Symbol:
POLQ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_955452.3
Protein GI Number:
139394648
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Swissprot #:
O75417