POU5F1B Antibody
| Code | Size | Price |
|---|
| PSI-42-581-0.1mg | 0.1mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
POU5F1B, POU class 5 homeobox 1B, OCT4-pg1, OTF3C, OTF3P1, POU5F1P1, POU5FLC20, POU5FLC8, POU 5 domain protein, POU class 5 homeobox 1 pseudogene 1, POU domain transcription factor Oct-4, POU domain, class 5, transcription factor 1 pseudogene 1, octamer binding protein 3_like sequence
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.6kDa according to NP_001153014.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-TPGYGSPHFTALYS
NCBI Gene ID #:
5462; 5460
NCBI Official Name:
POU class 5 homeobox 1B
NCBI Official Symbol:
POU5F1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001153014.1
Protein GI Number:
227430410
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Transcription
SPECIFICITY:
This antibody is expected to crossreact to the almost identical OCT4 / POU5F1 (GeneID 5460).
Swissprot #:
Q06416