MCP-1/MCAF Antibody

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ProSci
Product Code: PSI-XP-5219Bt
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5219Bt-0.05mg0.05mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
MCP-1/MCAF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated fr

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To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Rat MCP-1(MCAF) (XP-5219) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Rat MCP-1(MCAF).
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To detect Rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1(MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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To detect Rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1(MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Rat MCP-1(MCAF) (XP-5219) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Rat MCP-1(MCAF).
To detect Rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1(MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1(MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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Further Information

Additional Names:
MCP-1, Scya2, Sigje, Je, Mcp1, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, MCP-1
Application Note:
ELISA:

Sandwich:
To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat MCP-1(MCAF) (XP-5219) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1(MCAF).

Western Blot:
To detect rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat MCP-1(MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat MCP-1(MCAF) (rat MCP-1(MCAF)).
NCBI Gene ID #:
24770
NCBI Official Name:
chemokine (C-C motif) ligand 2
NCBI Official Symbol:
Ccl2
NCBI Organism:
Rattus norvegicustus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P14844
Protein GI Number:
126846
Purification:
Anti-rat MCP-1(MCAF) specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P14844
User NOte:
Centrifuge vial prior to opening.