PTPN11 Antibody

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ProSci
Product Code: PSI-46-250
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-250-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-250 staining (2ug/ml) of Human Muscle lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
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46-250 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stai
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46-250 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
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46-250 staining (2ug/ml) of Human Muscle lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
46-250 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stai
46-250 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Documents

Further Information

Additional Names:
PTPN11, SHP2, protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1), CFC, NS1, BPTP3, PTP2C, SHP-2, PTP-1D, SH-PTP2, SH-PTP3, Noonan syndrome 1, protein-tyrosine phosphatase 2C, protein tyrosine phosphatase, non-receptor type 11, MGC14433, protein tyrosine phosphatase-2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in Human Muscle lysates (calculated MW of 68.0kDa according to NP_002825.3). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Background References:
  • Saxton TM, Ciruna BG, Holmyard D, Kulkarni S, Harpal K, Rossant J, Pawson T. The SH2 tyrosine phosphatase shp2 is required for mammalian limb development. Nat Genet 2000 Apr;24(4):420-3
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-YENVGLMQQQKSFR
NCBI Gene ID #:
5781
NCBI Official Name:
protein tyrosine phosphatase, non-receptor type 11
NCBI Official Symbol:
PTPN11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002825.3, NP_001317366.1
Protein GI Number:
33356177
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Infectious Disease
SPECIFICITY:
.
Swissprot #:
Q06124