IKBKE Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-177-0.1mL | 0.1mL | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 6B4B5
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
IKBKE monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
IKKE, IKKI, KIAA0151, Inducible I kappa-B kinase, I-kappa-B kinase epsilon, IKKE, IKK-E, IKK-i
Application Note:
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon.The transcription factor NF?B is retained in the cytoplasm in an inactive form by the inhibitory protein I?B. Activation of NF?B requires that I?B be phosphorylated on specific serine residues, which results in targeted degra-dation of I?B. I?B kinase α(IKKα), previously designated CHUK, interacts with I?B-αand specifically phosphorylates I?B-αon the sites that trigger its degradation, serines 32 and 36. The functional IKK complex contains three subunits, IKKα, IKKβand IKK?(also designated NEMO), and each appear to make essential contributions to I?B phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKKαand IKKβ. IKK-i is pri-marily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNFα, IL-1 and IL-6. Overexpression of IKK-i was shown to result in phosphorylation of I?Bαon Ser32 and Ser36, and in NF?B activation, suggesting that IKK-i may act as an I?B kinase in the immune system.
Background References:
- Cell. 2007 Jun 15;129(6):1065-79.
- Mol Syst Biol. 2007;3:89. Epub 2007 Mar 13.
- Arthritis Rheum. 2007 Mar;56(3):743-52.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Purified truncated recombinant IKBKE-His (aa1-257) is expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
9641
NCBI Official Name:
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
NCBI Official Symbol:
IKBKE
NCBI Organism:
Homo sapiens
Protein Accession #:
Q14164
Protein GI Number:
14548079
Research Area:
Innate Immunity ,Signal Transduction
Swissprot #:
Q14164
User NOte:
Optimal dilutions for each application to be determined by the researcher.