RIF1 Antibody

ProSci
Product Code: PSI-46-300
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-300-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-300 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-300 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
Rif1, telomere-associated protein RIF1 homolog, Rap1 interacting factor 1, RAP1 interacting factor homolog (yeast), DKFZp434D1026, DKFZp781N1478, FLJ12870, OTTHUMP00000162712, RAP1 interacting factor 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 247kDa according to NP_060621. Therefore we cannot recommend an optimal concentration.Immunohistochemistry:Paraffin embedded Human Testis and Brain (Cortex). Recommended concentration: 1-2ug/ml.
Background References:
  • Silverman J, Takai H, Buonomo SB, Eisenhaber F, de Lange T. Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint. Genes Dev. 2004 Sep 1;18(17):2108-19.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: CEEGIIDANKTETNT
NCBI Gene ID #:
55183
NCBI Official Name:
RAP1 interacting factor homolog (yeast)
NCBI Official Symbol:
RIF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060621.3
Protein GI Number:
56676335
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise an epitope corresponding to aa 2076-2089 of human RIF1 protein.
Swissprot #:
Q5UIP0