Robo1 Antibody

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ProSci
Product Code: PSI-46-315
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-315-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-315 (1ug/ml) staining of HUVEC lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-315 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black lin
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46-315 (1ug/ml) staining of HUVEC lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-315 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black lin

Documents

Further Information

Additional Names:
ROBO1, DUTT1, roundabout, axon guidance receptor, homolog 1 (Drosophila) , HGNC:10249, FLJ21882, SAX3, axon guidance receptor, roundabout 1, MGC131599, MGC133277
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:An anonymous source observed an approx 250kDa band in Human Endothelial cell lysates at 1ug/ml antibody concentration, thought to be due to glycosylation. Some proteolytic breakdown products were also seen. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 2ug/ml.Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Background References:
  • Hannula-Jouppi K, Kaminen-Ahola N, Taipale M, Eklund R, Nopola-Hemmi J, Kaariainen H, Kere J. The Axon Guidance Receptor Gene ROBO1 Is a Candidate Gene for Developmental Dyslexia. PLoS Genet. 2005 Oct;1(4):e50. Epub 2005 Oct 28.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-GDVDLSNKINEMK
NCBI Gene ID #:
6091
NCBI Official Name:
roundabout, axon guidance receptor, homolog 1 (Drosophila)
NCBI Official Symbol:
ROBO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002932.1, NP_598334.1
Protein GI Number:
4506569
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Not expected to cross-react with ROBO2 or ROBO3.
Swissprot #:
Q9Y6N7