SATB1 Antibody

ProSci
Product Code: PSI-42-764
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-764-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-764 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-764 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
SATB1, SATB homeobox 1, DNA-binding protein SATB1, special AT-rich sequence binding protein 1, special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA's)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Experiments gave bands at approx. 110kDa and 85kDa in lysates of cell line MOLT4 after 0.3ug/ml antibody staining. The observed 110kDa band corresponds to earlier findings in literature with different antibodies (De Belle et al, J Cell Biol. 1998 Apr 20;141(2):335-48.; PMID: 9548713). This protein has a calculated MW of 86.0kDa according to NP_002962.1. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Thymus shows select nuclear staining. Recommended concentration, 3-5ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-ESDEENRQKTRPRT
NCBI Gene ID #:
6304
NCBI Official Name:
SATB homeobox 1
NCBI Official Symbol:
SATB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002962.1
Protein GI Number:
4506791
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
Reported variants NP_001124482.1 and NP_002962.1 represent identical protein.
Swissprot #:
Q01826