SIRT4 Antibody

ProSci
Product Code: PSI-46-373
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-373-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-373 (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-373 (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
SIRT4, SIR2L4, sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae), sirtuin silent mating type information regulation 2 homolog 4 (S. cerevisiae), MGC130046, MGC130047, MGC57437, SIR2L4, sirtuin 4, sir2-like 4, sirtuin 4, sirtuin type 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 38kDa band observed in Human Kidney lysates (calculated MW of 35.2kDa according to NP_036372.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section. Recommended concentration: 2-3ug/ml.
Background References:
  • Frye RA. Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity. Biochem Biophys Res Commun. 1999 Jun 24;260(1):273-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: SRCGELLPLIDPC
NCBI Gene ID #:
23409
NCBI Official Name:
sirtuin 4
NCBI Official Symbol:
SIRT4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036372.1
Protein GI Number:
6912662
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9Y6E7