SLC10A2 Antibody
| Code | Size | Price |
|---|
| PSI-42-041-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
SLC10A2, ASBT, solute carrier family 10 (sodium/bile acid cotransporter family), member 2, ISBT, NTCP2, OTTHUMP00000040691, ileal apical sodium-dependent bile acid transporter, ileal sodium-dependent bile acid transporter
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx. 60kDa band in Human Kidney lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 37.7kDa according to NP_000443.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Small Intestine shows staining of cells in the laminia propria between the intestinal vili. Recommended concentration, 3-6ug/ml.
Background References:
- Annaba F, Sarwar Z, Kumar P, Saksena S, Turner JR, Dudeja PK, Gill RK, Alrefai WA. Modulation of ileal bile acid transporter (ASBT) activity by depletion of plasma membrane cholesterol: association with lipid rafts. Am J Physiol Gastrointest Liver Physiol. 2008 Feb;294(2):G489-97. Epub 2007 Dec 6.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-YKANGGFQPDEK
NCBI Gene ID #:
6555
NCBI Official Name:
solute carrier family 10 (sodium/bile acid cotransporter), member 2
NCBI Official Symbol:
SLC10A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000443.1
Protein GI Number:
4506973
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Cancer
Swissprot #:
Q12908