SREC Antibody

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ProSci
Product Code: PSI-46-433
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-433-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-433 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.
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46-433 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
SCARF1, SREC, KIAA0149, MGC47738, scavenger receptor class F, member 1, acetyl LDL receptor, scavenger receptor expressed by endothelial cells
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 85-90kDa band observed in Human Heart lysates (calculated MW of 87.4kDa according to NP_003684.2). Recommended concentration: 0.3-1.0ug/ml. Primary incubation was 1 hour. An additional faint band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal.
Background References:
  • Adachi H, Tsujimoto M, Arai H, Inoue K. Expression cloning of a novel scavenger receptor from human endothelial cells. J Biol Chem. 1997 Dec 12;272(50):31217-20.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: SELDPKGQHVC
NCBI Gene ID #:
8578
NCBI Official Name:
scavenger receptor class F, member 1
NCBI Official Symbol:
SCARF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003684.2, NP_663325.1, NP_663327.2
Protein GI Number:
33598929
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise all three reported isoforms (NP_003684.2; NP_663325.1; NP_663327.2). Epitope corresponding to aa 20-31, mapping to the mature human SCARF1 / SREC1 protein.
Swissprot #:
Q14162