SYT1 Antibody

ProSci
Product Code: PSI-42-741
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-741-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-741 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-741 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
synaptotagmin I , DKFZp781D2042, SYT1, P65, SVP65, SYT, synaptotagmin 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 65kDa band observed in Human Brain (hippocampus) and Mouse and Rat Brain lysates (calculated MW of 47.6kDa according to NP_005630.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Matthew et al, J Cell Biol. 1981 Oct;91(1):257-69.; PMID: 7298720). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Brain shows vesicular staining in neuronal cell and axon. Recommended concentration: 4-6ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RDLQSAEKEEQEK
NCBI Gene ID #:
6857
NCBI Official Name:
synaptotagmin I
NCBI Official Symbol:
SYT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005630.1
Protein GI Number:
5032139
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
Reported isoforms NP_005630.1, NP_001129277.1 and NP_001129278.1 represent identical protein.
Swissprot #:
P21579