TPPP Antibody

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ProSci
Product Code: PSI-42-789
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-789-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-789 (0.001ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-789 Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
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42-789 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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42-789 (0.001ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-789 Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
42-789 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
TPPP, tubulin polymerization promoting protein, TPPP/p25, TPPP1, p24, p25, p25alpha, brain specific protein p25 alpha, glycogen synthase kinase 3 (GSK3) inhibitor p24, tubulin polymerization-promoting protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313(20):4091-106; PMID: 18028908). Recommended concentration: 0.001-0.01ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2ug/ml. Paraffin embedded Human Cortex. Recommended concentration: 3.75ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DPSKDRAAKRLSLES
NCBI Gene ID #:
11076
NCBI Official Name:
tubulin polymerization promoting protein
NCBI Official Symbol:
TPPP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_008961.1
Protein GI Number:
5902018
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Swissprot #:
O94811