Tspo Antibody

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ProSci
Product Code: PSI-42-086
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-086-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-086 (3.75ug/ml) staining of paraffin embedded Human Renal Tubules. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
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42-086 (2ug/ml) staining of NIH3T3 (A) amd Rat Adrenal Gland (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-086 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial /cytoplasmic staining. The nuclear
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42-086 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
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42-086 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
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42-086 (3.75ug/ml) staining of paraffin embedded Human Renal Tubules. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
42-086 (2ug/ml) staining of NIH3T3 (A) amd Rat Adrenal Gland (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-086 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial /cytoplasmic staining. The nuclear
42-086 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
42-086 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f

Documents

Further Information

Additional Names:
PBR, Tspo, translocator protein, Bzrp, IBP, benzodiazepine receptor, peripheral, isoquinoline-binding protein, peripheral benzodiazepine receptor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx.17+18kDa doublet band observed in cell lysates of NIH3T3 and in Rat Adrenal Gland lysates (calculated MW of 18.8kDa according to Mouse NP_033905.3 and 18.9kDa according to Rat NP_036647.1). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 and HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.

Background References:
  • Weisinger G, Kelly-Hershkovitz E, Veenman L, Spanier I, Leschiner S, Gavish M. Peripheral benzodiazepine receptor antisense knockout increases tumorigenicity of MA-10 Leydig cells in vivo and in vitro. Biochemistry 2004 Sep 43 (38): 12315-21.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-RDNSGRRGGSRLPE
NCBI Gene ID #:
12257 (mouse); 24230 (rat);
NCBI Official Name:
translocator protein
NCBI Official Symbol:
Tspo
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_033905.3
Protein GI Number:
163838662
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
P50637