VPS35 Antibody

Contact us
ProSci
Product Code: PSI-46-584
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-584-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 4
46-584 (1ug/ml) staining of Human Cerebellum (A) and Mouse Brain (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
2 / 4
3 / 4
4 / 4
46-584 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
❮❯

46-584 (1ug/ml) staining of Human Cerebellum (A) and Mouse Brain (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-584 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b

Documents

Further Information

Additional Names:
VPS35, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672, vacuolar protein sorting 35 (yeast), maternal-embryonic 3, vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting 35
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 90kDa band observed in Human Brain (Cerebellum), Human Heart and Human Ovary and Mouse Brain lysates (calculated MW of 91.7kDa according to NP_060676.2). Recommended concentration: 1.0-3.0ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Brain for this particular batch.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OScells. Recommended concentration: 10ug/ml.
Background References:
  • Zhang P, Yu L, Gao J, Fu Q, Dai F, Zhao Y, Zheng L, Zhao S. Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21. Genomics. 2000 Dec 1;70(2):253-7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: C-SPESEGPIYEGLIL
NCBI Gene ID #:
55737
NCBI Official Name:
vacuolar protein sorting 35 homolog (S. cerevisiae)
NCBI Official Symbol:
VPS35
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060676.2
Protein GI Number:
17999541
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
Swissprot #:
Q96QK1