Carabin Antibody

ProSci
Product Code: PSI-46-638
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-638-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-638 (0.5ug/ml) staining of Jurkat (A), (0.3ug/ml) PBM (B) and negative control A431 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-638 (0.5ug/ml) staining of Jurkat (A), (0.3ug/ml) PBM (B) and negative control A431 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
TBC1D10C, carabin, TBC1 domain family, member 10C, FLJ00332, MGC46488
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48-50kDa band observed in lysates of cell line Jurkat and in Mouse Thymus lysates and approx 50kDa in Peripheral Blood Mononuclear (PBM) and in Human Spleen lysates (calculated MW of 49.7Da according to Human NP_940919.1 and 49.9kDa according to Mouse NP_848765.2). Recommended concentration:0.3-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 cell lysate.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Background References:
  • Pan F, Sun L, Kardian DB, Whartenby KA, Pardoll DM, Liu JO. Feedback inhibition of calcineurin and Ras by a dual inhibitory protein Carabin. Nature. 2007 Jan 25;445(7126):433-6. Epub 2007 Jan 17.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-PRRKPQTRGKTFH
NCBI Gene ID #:
374403
NCBI Official Name:
TBC1 domain family, member 10C
NCBI Official Symbol:
TBC1D10C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_940919.1
Protein GI Number:
38348348
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
Q8IV04