NPFFR1 Antibody

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ProSci
Product Code: PSI-46-695
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-695-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-695 (0.03ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-695 (0.03ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
NPFFR1, neuropeptide FF receptor 1 , FLJ10751, GPR147, NPFF1, NPFF1R1, OT7T022, G protein-coupled receptor 147, neuropeptide FF 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 47.8kDa according to NP_071429.1). Recommended concentration: 0.03-0.1ug/ml.
Background References:
  • Gouarderes C, Mazarguil H, Mollereau C, Chartrel N, Leprince J, Vaudry H, Zajac JM. Functional differences between NPFF1 and NPFF2 receptor coupling: high intrinsic activities of RFamide-related peptides on stimulation of [35S]GTPgammaS binding. Neuropharmacology. 2007 Feb;52(2):376-86. Epub 2006 Sep 29.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-RPSGSHKEAYSERP
NCBI Gene ID #:
64106
NCBI Official Name:
neuropeptide FF receptor 1
NCBI Official Symbol:
NPFFR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_071429.1
Protein GI Number:
11545887
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
Q9GZQ6