RPS19 Antibody

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ProSci
Product Code: PSI-46-322
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-322-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-322 (0.3ug/ml) staining of lysate of cell line K562 (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-322 (5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-322 (0.3ug/ml) staining of lysate of cell line K562 (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-322 (5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
RPS19, ribosomal protein S19, HGNC:10402, DBA, 40S ribosomal protein S19
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 16kDa band observed in lysate of cell line K562 (calculated MW of 16.1kDa according to NP_001013.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex), Liver and Tonsil. Recommended concentration: 5ug/ml.
Background References:
  • Leger-Silvestre I, Caffrey JM, Dawaliby R, Alvarez-Arias DA, Gas N, Bertolone SJ, Gleizes PE, Ellis SR. Specific role for yeast homologs of the diamond blackfan anemia associated Rps19 protein in ribosome synthesis. J Biol Chem. 2005 Sep 12;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KMVEKDQDGGRK
NCBI Gene ID #:
6223
NCBI Official Name:
ribosomal protein S19
NCBI Official Symbol:
RPS19
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001013.1
Protein GI Number:
4506695
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P39019