P114-RHO-GEF Antibody

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ProSci
Product Code: PSI-46-122
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-122-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-122 (0.5ug/ml) staining of lysates of cell lines Daudi (A) and Caco-2 (B) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-122 (0.5ug/ml) staining of lysates of cell lines Daudi (A) and Caco-2 (B) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ARHGEF18, Rho/Rac guanine nucleotide exchange factor (GEF) 18, P114-RHO-GEF, KIAA0521, MGC15913, Rho-specific guanine nucleotide exchange factor p114, P114-RhoGEF, Rho/Rac guanine nucleotide exchange factor 18
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 140+90kDa band observed in lysates of cell line Daudi and Caco-2 (calculated MW of 114kDa according to NP_056133.2 and 131kDa accoeding to NP_001124427.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Blomquist A, Schworer G, Schablowski H, Psoma A, Lehnen M, Jakobs KH, Rumenapp U. Identification and characterization of a novel Rho-specific guanine nucleotide exchange factor. Biochem J. 2000 Dec 1;352 Pt 2:319-25.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-SAKEDASKEDVIFF
NCBI Gene ID #:
23370
NCBI Official Name:
Rho/Rac guanine nucleotide exchange factor (GEF) 18
NCBI Official Symbol:
ARHGEF18
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_056133.2, NP_001124427.1
Protein GI Number:
41327769
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1).
Swissprot #:
Q6ZSZ5