TRAP2 Antibody

ProSci
Product Code: PSI-46-512
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-512-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-512 (1 ug/ml) staining of HeLa (A), U251 (B), KNRK (C) and (0.3ug/ml) NIH3T3 (D) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

46-512 (1 ug/ml) staining of HeLa (A), U251 (B), KNRK (C) and (0.3ug/ml) NIH3T3 (D) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
PSMD2, TRAP2, MGC14274, S2, P97, proteasome (prosome, macropain) 26S subunit, non-ATPase, 2, 55.11 protein, TNFR-associated protein 2, 26S proteasome subunit p97, 26S proteasome regulatory subunit S2, 26S proteasome non-ATPase regulatory subunit 2, tumor necrosis factor receptor-associated protein 2, Rpn1, proteasome 26S non-ATPase subunit 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 100kDa band observed in lysates of cell lines HeLa, U251, NIH3T3 and KNRK (calculated MW of 100kDa according to Human NP_002799.3, Mouse NP_598862.1 and Rat NP_001026809.1). Recommended concentration: 0.3-1?g/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10?g/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Background References:
  • Kuai J, Wooters J, Hall JP, Rao VR, Nickbarg E, Li B, Chatterjee-Kishore M, Qiu Y, Lin LL. NAK is recruited to the TNFR1 complex in a TNFalpha-dependent manner and mediates the production of RANTES: identification of endogenous TNFR-interacting proteins by a proteomic approach. J Biol Chem. 2004 Dec 17;279(51):53266-71. Epub 2004 Oct 13.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-VILRKNPNYDL
NCBI Gene ID #:
5708
NCBI Official Name:
proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
NCBI Official Symbol:
PSMD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002799.3
Protein GI Number:
25777602
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q13200