EEF1A1 Antibody

ProSci
Product Code: PSI-30-369
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-30-369-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EEF1A1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.

Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.

Further Information

Additional Names:
EEF1A1, CCS-3, CCS3, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, LENG7, MGC102687, MGC131894, MGC16224, PTI1, eEF1A-1, EE1A1
Application Note:
EEF1A1 antibody can be used for detection of EEF1A1 by ELISA at 1:12500. EEF1A1 antibody can be used for detection of EEF1A1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background References:
  • Zhang, Y., (2008) Biochem. Biophys. Res. Commun. 369 (4), 1057-1060.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EEF1A1.
NCBI Gene ID #:
1915
NCBI Official Name:
eukaryotic translation elongation factor 1 alpha 1
NCBI Official Symbol:
EEF1A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
NP_001393
Protein GI Number:
4503471
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Cancer
Swissprot #:
P68104
User NOte:
Optimal dilutions for each application to be determined by the researcher.