SIGLEC12 Antibody
| Code | Size | Price |
|---|
| PSI-30-597-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SIGLEC12 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
SIGLEC12, FLJ38600, S2V, SIGLECL1, SLG, Siglec-12, Siglec-L1, Siglec-XII
Application Note:
SIGLEC12 antibody can be used for detection of SIGLEC12 by ELISA at 1:312500. SIGLEC12 antibody can be used for detection of SIGLEC12 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. SIGLEC12 is a member of the SIGLEC3-like subfamily of SIGLECs. SIGLEC12, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor.Western blots using four different antibodies against four unique regions of this protein target confirm the same apparent molecular weight in our tests.Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene.
Background References:
- Angata, T., (2001) J. Biol. Chem. 276 (43), 40282-40287.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIGLEC12.
NCBI Gene ID #:
89858
NCBI Official Name:
sialic acid binding Ig-like lectin 12 (gene/pseudogene)
NCBI Official Symbol:
SIGLEC12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
63 kDa
Protein Accession #:
NP_443729
Protein GI Number:
16506828
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Membrane ,Signal Transduction
Swissprot #:
Q96PQ1
User NOte:
Optimal dilutions for each application to be determined by the researcher.