BAG4 Antibody
| Code | Size | Price |
|---|
| PSI-46-977-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
BAG4, BCL2-associated athanogene 4, BAG-4, SODD, BAG-family molecular chaperone regulator-4, silencer of death domains
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 60kDa band observed in cell lines HEK293 and HeLa lysates (calculated MW of 49.6kDa according to NP_004865.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Takada et al, Mol Cell Biol. 2003 Jun;23(11):4026-33.; PMID: 12748303). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.
An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml.
Background References:
- Annunziata CM, Kleinberg L, Davidson B, Berner A, Gius D, Tchabo N, Steinberg SM, Kohn EC. BAG-4/SODD and associated antiapoptotic proteins are linked to aggressiveness of epithelial ovarian cancer. Clin Cancer Res. 2007 Nov 15;13(22 Pt 1):6585-92
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-GNATSDHPNNQDQSS
NCBI Gene ID #:
9530
NCBI Official Name:
BCL2-associated athanogene 4
NCBI Official Symbol:
BAG4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004865.1
Protein GI Number:
6631075
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Apoptosis
SPECIFICITY:
.
Swissprot #:
O95429