CHN2 Antibody
| Code | Size | Price |
|---|
| PSI-42-020-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
CHN2, chimerin (chimaerin) 2, ARHGAP3, RHOGAP3, RhoGAP3, beta chimerin, Chimerin 2 (GTPase-activating protein, rho, 3), BCH, MGC138360, rho-GTPase-activating protein 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments in Human Brain (Cerebellum and Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Cortex shows staining of neuronal bodies. Recommended concentration, 3-6ug/ml.
Background References:
- Siliceo, M. and Merida, I. T cell receptor-dependent tyrosine phosphorylation of beta2-chimaerin modulates its Rac-GAP function in T cells. J. Biol. Chem. 284 (17), 11354-11363 (2009).
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QILIENEDVLF
NCBI Gene ID #:
1124
NCBI Official Name:
chimerin 2
NCBI Official Symbol:
CHN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001035025.1, NP_004058.1
Protein GI Number:
91106928
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_004058.1; NP_001035025.1).
Swissprot #:
P52757