MAN2A1 Antibody

ProSci
Product Code: PSI-45-861
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-861-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-861 (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-861 (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
MAN2A1, mannosidase, alpha, class 2A, member 1, GOLIM7, MANA2, MANII, Golgi alpha-mannosidase II, golgi integral membrane protein 7, mannosidase, alpha type II, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 150kDa band observed in Human Placenta lysates (calculated MW of 131kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx. 98kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml. An anonymous customer found positive results in WB on Human dermal fibroblasts.
Background References:
  • Green RS, Stone EL, Tenno M, Lehtonen E, Farquhar MG, Marth JD. Mammalian N-glycan branching protects against innate immune self-recognition and inflammation in autoimmune disease pathogenesis. Immunity. 2007 Aug;27(2):308-20. Epub 2007 Aug 2.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-KISSDIKSQNR
NCBI Gene ID #:
4124
NCBI Official Name:
mannosidase, alpha, class 2A, member 1
NCBI Official Symbol:
MAN2A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002363.2
Protein GI Number:
51477714
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q16706