Persephin Antibody

ProSci
Product Code: PSI-38-119
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-119-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Persephin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated fre

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To detect hPersephin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human Persephin (38-120) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin.

To detect hPersephin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human Persephin (38-120) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin.

Further Information

Additional Names:
PSPPersephinPSP
Application Note:
ELISA:
Indirect:
To detect hPersephin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin.

Sandwich
To detect hPersephin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Persephin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin.

Western Blot:
To detect hPersephin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPersephin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Persephin signals through a multicomponent receptor system, composed of RET and one of four GFR alpha (alpha1-alpha4) receptors. The GFRalpha4 was first identified in chicken and was later shown to be the preferential binding subunit for Persephin. In contrast to GDNF and Neurturin, Persephin does not support survival of peripheral neurons.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPersephin. Human Persephin specific antibody was purified by affinity chromatography employing immobilized hPersephin matrix.
NCBI Gene ID #:
5623
NCBI Official Name:
persephin
NCBI Official Symbol:
PSPN
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
O60542
Protein GI Number:
7531178
Research Area:
Neuroscience,Antibody Pairs
Swissprot #:
O60542
User NOte:
Centrifuge vial prior to opening.