PTCH Antibody

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ProSci
Product Code: PSI-46-245
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-245-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-245 (3.75ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-245 (3.75ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
PTCH1, PTCH, patched homolog (Drosophila), HGNC:9585, BCNS, HPE7, NBCCS, PTC, PTC1, patched, patched (Drosophila) homolog, patched homolog 1 (Drosophila), RP11-435O5.3, FLJ26746, FLJ42602, PTCH11, OTTHUMP00000021709, OTTHUMP00000021710, PTCH protein +12b, PTCH protein +4', PTCH protein -10
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Immunohistochemistry:In paraffin embedded Human Colon shows textured nuclear staining of the absorptive cells. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Breast. Recommended concentration: 3.75ug/ml. This produt has been successfully used in IHC on Human: Abiria SA et al, J Neurooncol. 2014 May 28. PMID: 24867209.Immunofluorescence: Expression of the protein seen in the cytoplasm of HeLa and in the Golgi/membranes of NIH3T3 cells. Recommended concentration: 5ug/ml. This produt has been successfully used in IF on Human: Abiria SA et al, J Neurooncol. 2014 May 28. PMID: 24867209.
Background References:
  • Nagao K, Togawa N, Fujii K, Uchikawa H, Kohno Y, Yamada M, Miyashita T. Detecting tissue-specific alternative splicing and disease-associated aberrant splicing of the PTCH gene with exon junction microarrays. Hum Mol Genet. 2005 Oct 3;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: ELQDVECEERPR
NCBI Gene ID #:
5727
NCBI Official Name:
patched 1
NCBI Official Symbol:
PTCH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000255.1, NP_001077072.1, NP_001077071.1, NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1
Protein GI Number:
4506247
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.
Swissprot #:
Q13635