SLC7A11 Antibody

Contact us
ProSci
Product Code: PSI-46-378
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-378-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 6
46-378 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
2 / 6
3 / 6
4 / 6
46-378 (0.1ug/ml) staining of Human Smooth Muscle (A) and Tonsil (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
5 / 6
46-378 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle/cytoplasmic staining. The nuclear stain is
6 / 6
46-378 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
❮❯

46-378 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-378 (0.1ug/ml) staining of Human Smooth Muscle (A) and Tonsil (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-378 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle/cytoplasmic staining. The nuclear stain is
46-378 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Documents

Further Information

Additional Names:
SLC7A11, solute carrier family 7, (cationic amino acid transporter, y+ system) member 11, CCBR1, xCT , cystine/glutamate transporter
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50-55kDa band observed in lysates of cell lines A549 and U2OS, and in Human Smooth Muscle and Tonsil lysates (calculated MW of 55.4kDa according to NP_055146.1). Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the vesicles/ cytoplasm of A549 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Background References:
  • Kaleeba JA, Berger EA. Kaposi's sarcoma-associated herpesvirus fusion-entry receptor: cystine transporter xCT. Science. 2006 Mar 31;311(5769):1921-4.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-KGQTQNFKDAFSGRD
NCBI Gene ID #:
23657
NCBI Official Name:
solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11
NCBI Official Symbol:
SLC7A11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055146.1
Protein GI Number:
7657683
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9UPY5