PITPNM3 Antibody

ProSci
Product Code: PSI-46-196
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-196-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-196 (5ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

46-196 (5ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
PITPNM3, NIR1, PITPNM family member 3, PYK2 N-terminal domain-interacting receptor 1, RDGBA3, CORD5, MGC157740, MGC157741, membrane-associated phosphatidylinositol transfer protein 3, retinal degeneration B alpha 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 50kDa band in Human Heart lysates after 0.1ug/ml antibody staining. The 50kDa band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kDa according to NP_112497. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows strong staining of cytoplasm in several neuronal cells. Recommended concentration, 5-10ug/ml.
Background References:
  • Lev S, Hernandez J, Martinez R, Chen A, Plowman G, Schlessinger J. Identification of a novel family of targets of PYK2 related to Drosophila retinal degeneration B (rdgB) protein. Mol Cell Biol. 1999 Mar;19(3):2278-88.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-SWARGPPKFESVP
NCBI Gene ID #:
83394
NCBI Official Name:
PITPNM family member 3
NCBI Official Symbol:
PITPNM3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_112497.2, NP_001159438.1
Protein GI Number:
190358515
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9BZ71