PODXL Antibody

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ProSci
Product Code: PSI-46-206
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-206-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-206 (1ug/ml) staining of Human Kisney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-206 (1ug/ml) staining of Human Kisney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PODXL, podocalyxin-like, Gp200, MGC138240, PCLP
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 160kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 58.6kDa according to NP_001018121.1 and 55.4kDa according to NP_005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kershaw et al., J Biol Chem. 1997 Jun 20;272(25):15708-14.; PMID: 9188463). Recommended concentration: 1-3ug/ml.
Background References:
  • Nielsen JS, Graves ML, Chelliah S, Vogl AW, Roskelley CD, McNagny KM. The CD34-Related Molecule Podocalyxin Is a Potent Inducer of Microvillus Formation. PLoS ONE. 2007 Feb 21;2:e237.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-DNLTKDDLDEEEDTH
NCBI Gene ID #:
5420
NCBI Official Name:
podocalyxin-like
NCBI Official Symbol:
PODXL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001018121.1 , NP_005388.2
Protein GI Number:
66277202
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_001018121.1 and NP_005388.2).
Swissprot #:
O00592