TRIM4 Antibody

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ProSci
Product Code: PSI-46-525
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-525-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-525 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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46-525 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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Further Information

Additional Names:
TRIM4, RNF87, tripartite motif-containing 4, tripartite motif protein 4, tripartite motif protein TRIM4, tripartite motif protein TRIM4 isoform alpha, tripartite motif protein 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55-60kDa band observed in HepG2 whole cell lysate (predicted MW of 58kDa and 55kDa according to NP_148977 and NP_149082). Recommended for use at 1ug/ml.
Background References:
  • Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A. The tripartite motif family identifies cell compartments. EMBO J. 2001 May 1;20(9):2140-51.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-ASLVIPPVTDRK
NCBI Gene ID #:
89122
NCBI Official Name:
tripartite motif containing 4
NCBI Official Symbol:
TRIM4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_148977.1, NP_149082.1
Protein GI Number:
301601615
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported human isoforms (as represented by NP_148977 and NP_149082).
Swissprot #:
Q9C037