PHB Antibody
| Code | Size | Price |
|---|
| PSI-27-716-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PHB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
PHB, PHB1, HEL-215, HEL-S-54e
Application Note:
PHB antibody can be used for detection of PHB by ELISA at 1:1562500. PHB antibody can be used for detection of PHB by western blot at 1.0-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
Background References:
- Fraering, P.C., et al., (2004) Biochemistry 43 (30), 9774-9789.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PHB.
NCBI Gene ID #:
5245
NCBI Official Name:
prohibitin
NCBI Official Symbol:
PHB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
NP_002625
Protein GI Number:
4505773
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription ,Cell Cycle ,Cancer
Swissprot #:
P35232
User NOte:
Optimal dilutions for each application to be determined by the researcher.