CD14 Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-128-0.1mL | 0.1mL | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 5A3H5D5FC
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
Storage:
CD14 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Myeloid cell-specific leucine-rich glycoprotein
Application Note:
IHC:Dilution 1:200 - 1:400
ELISA:Determining optimal working dilutions by titration test.
ELISA:Determining optimal working dilutions by titration test.
Background:
CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP). LBP and CD14 antigen serves two physiological roles. These proteins act as opsonin and opsonic receptor, respectively, to promote the phagocytic uptake of bacteria or LPScoated particles by macrophages.
Background References:
- J Mammary Gland Biol Neoplasia 2000, 5 : 227-241.
- J Mammary Gland Biol Neoplasia 2000, 5 : 165-185.
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant human CD14 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
929
NCBI Official Name:
CD14 molecule
NCBI Official Symbol:
CD14
NCBI Organism:
Homo sapiens
Protein Accession #:
P08571
Protein GI Number:
20141203
Purification:
Antibody is purified by protein G affinity chromatography and resuspended in PBS with 0.2%BSA,
0.09% sodium azide. No reconstitution is necessar Concentration is 0.4mg/ml.
0.09% sodium azide. No reconstitution is necessar Concentration is 0.4mg/ml.
Research Area:
Innate Immunity
Swissprot #:
P08571
User NOte:
Optimal dilutions for each application to be determined by the researcher.