BHMT Antibody

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ProSci
Product Code: PSI-46-634
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-634-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-634 (0.03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-634 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-634 (0.03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-634 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
BHMT, betaine-homocysteine methyltransferase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). This product has been successfully used in WB on Human (PMID: 27320863). This product has been successfully used in WB on Mouse (PMID:28605831, 23807810 and 22209966). This product has been successfully used in WB on Rat (Choi et al. Journal of Functional Foods 44 (2018) 65-73). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Kidney. Recommended concentration: 5ug/ml.
Background References:
  • Collinsova M, Strakova J, Jiracek J, Garrow TA. Inhibition of betaine-homocysteine S-methyltransferase causes hyperhomocysteinemia in mice. J Nutr. 2006 Jun;136(6):1493-7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-EQQLKELFEKQK
NCBI Gene ID #:
635
NCBI Official Name:
betaine--homocysteine S-methyltransferase
NCBI Official Symbol:
BHMT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001704.1
Protein GI Number:
4502407
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
Q93088