PPP4C Antibody

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ProSci
Product Code: PSI-42-003
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-003-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-003 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-003 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PPP4C, protein phosphatase 4 (formerly X), catalytic subunit, PP4, PPH3, PPX, Protein phosphatase 4, catalytic subunit
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 35kDa band observed in HEK293, A431 and HeLa lysates and in Rat Spleen and Lung lysates (calculated MW of 35.1kDa according to NP_002711.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Prostate shows nuclear staining of secretory cells in the gland. Recommended concentration, 2-4ug/ml.
Background References:
  • Mourtada-Maarabouni M, Williams GT. Protein phosphatase 4 regulates apoptosis, proliferation and mutation rate of human cells. Biochimica et biophysica acta 2008 Aug 1783 (8): 1490-502.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-PQETRGIPSKKP
NCBI Gene ID #:
5531
NCBI Official Name:
protein phosphatase 4, catalytic subunit
NCBI Official Symbol:
PPP4C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002711.1
Protein GI Number:
4506027
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
P60510