PSME2 Antibody

ProSci
Product Code: PSI-46-239
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-239-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-239 (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-239 (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PSME2, proteasome (prosome, macropain) activator subunit 2 (PA28 beta), PA28B, PA28beta, REGbeta , 11S regulator complex beta subunit, MCP activator, 31-kD subunit, activator of multicatalytic protease subunit 2, cell migration-inducing protein 22, proteasome activator 28-beta, proteasome activator hPA28 subunit beta, proteasome activator subunit 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 27kDa band observed in Human Spleen lysates (calculated MW of 27.4kDa according to NP_002809.2). Recommended concentration: 0.5-1ug/ml.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml.
Background References:
  • Brychcy M, Kuckelkorn U, Hausdorf G, Egerer K, Kloetzel PM, Burmester GR, Feist E. Anti-20S proteasome autoantibodies inhibit proteasome stimulation by proteasome activator PA28. Arthritis Rheum. 2006 Jul;54(7):2175-83.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-NLEKIVNPKGEEKP
NCBI Gene ID #:
5721
NCBI Official Name:
proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
NCBI Official Symbol:
PSME2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002809.2
Protein GI Number:
30410792
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9UL46