MLH3 Antibody

Contact us
ProSci
Product Code: PSI-45-889
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-889-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 2
45-889 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained wit
2 / 2
❮❯

45-889 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained wit

Documents

Further Information

Additional Names:
MLH3, mutL homolog 3 (E. coli), HNPCC, HNPCC7, MGC138372, S240II117, mutL (E. coli) homolog 3, mismatch repair gene MLH3, DNA mismatch repair protein MLH3, mutL homolog 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Background References:
  • Lipkin SM, Wang V, Jacoby R, Banerjee-Basu S, Baxevanis AD, Lynch HT, Elliott RM, Collins FS. MLH3: a DNA mismatch repair gene associated with mammalian microsatellite instability. Nat Genet. 2000 Jan;24(1):27-35.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: RQSLQQSMPPCEPP
NCBI Gene ID #:
27030
NCBI Official Name:
mutL homolog 3
NCBI Official Symbol:
MLH3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001035197.1, NP_055196.2
Protein GI Number:
91992162
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_001035197.1) and isoform 2 (NP_055196.2).
Swissprot #:
Q9UHC1