PPP2R4 Antibody

ProSci
Product Code: PSI-42-005
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-005-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-005 (0.5ug/ml) staining of Human Liver lysate (lane 1), Mouse Liver (lane 2) and Rat Liver (lane 3) (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-005 (0.5ug/ml) staining of Human Liver lysate (lane 1), Mouse Liver (lane 2) and Rat Liver (lane 3) (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
PPP2R4, protein phosphatase 2A activator, regulatory subunit 4, MGC2184, PP2A, PR53, PTPA, OTTHUMP00000022333, PP2A phosphatase activator, PP2A, subunit B', phosphotyrosyl phosphatase activator, protein phosphatase 2A, regulatory subunit B', protein phosphatase 2A, regulatory subunit B' (PR 53)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in Human, Mouse and Rat Liver lysates and in lysates of cell line HepG2 (calculated MW of 36.8kDa according to NP_066954.2). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 3.75ug/ml.
Background References:
  • Gushiken FC, Patel V, Liu Y, Pradhan S, Bergeron AL, Peng Y, Vijayan KV. Protein phosphatase 2A negatively regulates integrin alpha(IIb)beta(3) signaling. J Biol Chem 2008 May 283 (19): 12862-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-GSLLPIHPVTSG
NCBI Gene ID #:
5524
NCBI Official Name:
protein phosphatase 2A activator, regulatory subunit 4
NCBI Official Symbol:
PTPA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_821068.1, NP_066954.2, NP_821070.1
Protein GI Number:
30065645
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_821068.1; NP_066954.2; NP_821070.1). Reported variants NP_066954.2 and NP_821067.1 represent identical protein.
Swissprot #:
Q15257