CK1 Monoclonal Antibody

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ProSci
Product Code: PSI-32-137
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-137-0.1mL0.1mL£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3C10F7
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
CK1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using CK1 monoclonal antibody against truncated CK1 recombinant protein.
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Western blot analysis using CK1 monoclonal antibody against truncated CK1 recombinant protein.

Documents

Further Information

Additional Names:
CK1, CKI-alpha, CK1a, CKIa, HLCDGP1, PRO2975, HEL-S-77p
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
CK1 (also designated Cytokeratin 1 or KRT1 ),with 644-amino acid protein (about 70 kDa), is a member of the keratin family. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells.CK1 consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK1 is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.
Background References:
  • Ricardo G. Espinola, Robin A. Pixley, Berhane Ghebrehiwet. Blood (ASH Annual Meeting Abstracts), Nov 2005; 106: 2666.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant CK1 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
1452
NCBI Official Name:
casein kinase 1, alpha 1
NCBI Official Symbol:
CSNK1A1
NCBI Organism:
Homo sapiens
Protein Accession #:
P48729
Protein GI Number:
31077177
Swissprot #:
P48729
User NOte:
Optimal dilutions for each application to be determined by the researcher.