LAL Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-185-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 9G7F12
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
LAL monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Cholesteryl esterase, Acid cholesteryl ester hydrolase, LAL, CESD
Application Note:
Western Blot:Dilution 1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Lysosomal acid lipase (LAL), with 378-amino acid protein( 43-54 kDa), functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides which are taken up by receptor-mediated endocytosis. An inherited deficiency or low activity of human lysosomal acid lipase results in the intralysosomal storage of the respective lipid substrates. So it is also responsible for the rare conditions of Wolman disease and cholesteryl ester storage disease (CESD). As the enzyme is synthesized by all nucleated cells, lipid-laden cells are found in all organs, particularly in liver, spleen, the adrenal and the hemopoietic system, and in the intestine as well as in the lymph nodes, lungs, testes, and ovaries.
Background References:
- Uta Drebber, Matthias Andersen, Hans U Kasper, et al, World J Gastroenterol. 2005 Apr 21;11(15):2364-6.
- Renata Boldrini, Rita Devito, R.Biselli,et al, Pathol Res Pract. 2004;200(3):231-40.
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant LAL expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
3988
NCBI Official Name:
lipase A, lysosomal acid, cholesterol esterase
NCBI Official Symbol:
LIPA
NCBI Organism:
Homo sapiens
Protein Accession #:
P38571
Protein GI Number:
68067636
Purification:
Antibody is purified by protein A affinity chromatography.
Research Area:
Homeostasis
Swissprot #:
P38571
User NOte:
Optimal dilutions for each application to be determined by the researcher.