ORAI1 Antibody

ProSci
Product Code: PSI-46-765
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-765-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-765 (0.03ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-765 (0.03ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
ORAI1, CRACM1, ORAI calcium release-activated calcium modulator 1, FLJ14466, ORAT1, TMEM142A, calcium release-activated calcium channel protein 1, calcium release-activated calcium modulator 1, transmembrane protein 142A
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 50-55kDa band observed in Human Placenta and Skin lysates (calculated MW of 32.7kDa according to NP_116179.2). The observed molecular weight corresponds to different antibodies from other commercial sources and with earlier findings in literature (Li et al,; PMID: 17702753). Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Vig M, DeHaven WI, Bird GS, Billingsley JM, Wang H, Rao PE, Hutchings AB, Jouvin MH, Putney JW, Kinet JP. Defective mast cell effector functions in mice lacking the CRACM1 pore subunit of store-operated calcium release-activated calcium channels. Nat Immunol. 2008 Jan;9(1):89-96. Epub 2007 Dec 2.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: C-KKQPGQPRPTSKP
NCBI Gene ID #:
84876
NCBI Official Name:
ORAI calcium release-activated calcium modulator 1
NCBI Official Symbol:
ORAI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_116179.2
Protein GI Number:
38016943
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology
SPECIFICITY:
.
Swissprot #:
Q96D31