RPE65 Antibody
| Code | Size | Price |
|---|
| PSI-46-321-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
mRPE65, sRPE65, retinal pigment epithelium-specific protein 65kDa, LCA2, RP20, rd12, RBP-binding membrane protein, p63, retinal pigment epithelium-specific 61 kDa protein, retinal pigment epithelium-specific protein (65kD), retinitis pigmentosa 20
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band observed in Rat Retina lysates (calculated MW of 60.9kDa according to Human NP_000320.1 and 61.0kDa according to Rat NP_446014.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.
Background References:
- Rolling F, Le Meur G, Stieger K, Smith AJ, Weber M, Deschamps JY, Nivard D, Mendes-Madeira A, Provost N, Pereon Y, Cherel Y, Ali RR, Hamel C, Moullier P, Rolling F. Gene therapeutic prospects in early onset of severe retinal dystrophy: restoration of vision in RPE65 Briard dogs using an AAV serotype 4 vector that specifically targets the retinal pigmented epithelium. Bull Mem Acad R Med Belg. 2006;161(10-12):497-508; discussion 508-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog
Immunogen:
The immunogen for this antibody is: C-EVKKNARKAPQPE
NCBI Gene ID #:
6121
NCBI Official Name:
retinal pigment epithelium-specific protein 65kDa
NCBI Official Symbol:
RPE65
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000320.1
Protein GI Number:
4506591
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q16518