Adenosine A1 Receptor Antibody
| Code | Size | Price |
|---|
| PSI-46-622-0.1mg | 0.1mg | £565.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
ADORA1, adenosine A1 receptor, RDC7
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave bands at approx 30kDa and 20kDa in Human Brain (Cerebellum) lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 36.5kDa according to NP_000665.1 and NP_001041695.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
- Funakoshi H, Chan TO, Good JC, Libonati JR, Piuhola J, Chen X, MacDonnell SM, Lee LL, Herrmann DE, Zhang J, Martini J, Palmer TM, Sanbe A, Robbins J, Houser SR, Koch WJ, Feldman AM. Regulated overexpression of the A1-adenosine receptor in mice results in adverse but reversible changes in cardiac morphology and function. Circulation. 2006 Nov 21;114(21):2240-50. Epub 2006 Nov 6.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog, Cow
Immunogen:
The immunogen for this antibody is: NDHFRCQPAPPIDED
NCBI Gene ID #:
134
NCBI Official Name:
adenosine A1 receptor
NCBI Official Symbol:
ADORA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000665.1, NP_001041695.1
Protein GI Number:
4501947
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
Both reported isoforms represent identical protein (NP_000665.1; NP_001041695.1)
Swissprot #:
P30542